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1x phosphate buffered saline 1xpbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x phosphate buffered saline 1xpbs
    1x Phosphate Buffered Saline 1xpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+phosphate+buffered+saline+1xpbs/Phosphate-buffered+saline+(PBS%2C+10X)%2C+pH+7%2E4/pmc12965717-194-6-25
    Average 99 stars, based on 1 article reviews
    1x phosphate buffered saline 1xpbs - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila
    Article Snippet: .. 94512 13 of 17 dissected in 1x phosphate buffered saline (1xPBS) (Quality Biological, Gaithersburg, MD; 119- 069- 131) at room temperature and fixed in 4% paraformaldehyde (PFA) (Thermo Fisher Scientific; J19943. .. K2) in 1xPBS for 1 hour at room temperature, followed by three 15- minute washes in 1xPBS with Triton X- 100 (Sigma- Aldrich; 9002- 93- 1) (PBST).

    Article Title: Controllable Fusion of Human Brain Organoids Using Acoustofluidics
    Article Snippet: .. The fixed organoids were then washed twice in 1X phosphate-buffered saline (1XPBS) (Gibco) and transferred to 30% sucrose (w/v) to cryoprotect at 4°C overnight. ..

    Article Title: Controllable fusion of human brain organoids using acoustofluidics
    Article Snippet: .. The fixed organoids were then washed twice in 1X phosphate-buffered saline (1XPBS) (Gibco) and transferred to 30% sucrose (w/v) to cryoprotect at 4 °C overnight. ..

    Article Title: Integrating extracellular vesicle and circulating cell-free DNA analysis on a single plasma aliquot from breast cancer patients improves the detection of HER2 positivity
    Article Snippet: Primary antibodies used were rabbit anti -CD9 (#D801A; Cell signaling Technology; 1/500); rabbit anti – CD 9 (# MA5-33125; Invitrogen; 1/500); mouse anti – Apolipoprotein A1 (#5F4; Cell Signaling Technology; 1/250); rabbit anti– Albumin (#4929; Cell Signaling Technology; 1/1000), mouse anti-Tsg-101 (ab83, Abcam, 1/1000), rabbit anti-Flotillin 1 (#18634, Cell Signaling Technology; 1/1000), rabbit anti-Flotillin 2 (#3436, Cell Signaling Technology; 1/1000), rabbit anti-CD44 (ab157107, Abcam, 1/500), rabbit anti-EpCam (#D1B3, Cell Signaling Technology; 1/500), rabbit anti-HER2/ErbB2 (#29D8, Cell Signaling Technology; 1/1000). .. Isolated EV samples (10 10 -10 11 particles/mL) were resuspended in 1x Phosphate-Buffered Saline (1xPBS) (Gibco) and incubated overnight at 4C with HER2/ErbB2 (29D8) – PE-conjugated antibody (1:50) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (Cell Signaling Technology). .. EVs were then stained with one volume of CellMask plasma membrane stains (C10046, Life Technologies) diluted in 1xPBS (1:5000) for 15 minutes at room temperature, in the dark.

    Article Title: Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila
    Article Snippet: .. The flies were then dissected in 1x phosphate buffered saline (1xPBS) (Quality Biological, Gaithersburg, MD; 119-069-131) at room temperature and fixed in 4% paraformaldehyde (PFA) (Thermo Fisher Scientific; J19943.K2) in 1xPBS for 1 hour at room temperature, followed by three 15-minute washes in 1xPBS with Triton X-100 (Sigma-Aldrich; 9002-93-1) (PBST). .. The specimens were then stained in DAPI (0.5 μg/ml in PBST) (Thermo Fisher Scientific; D1306) for 10 minutes and washed once for 15 minutes in 1xPBS before mounting with Vectashield antifade mounting media (Vector Laboratories; H-1000).

    Article Title: Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila
    Article Snippet: .. The flies were then dissected in 1x phosphate buffered saline (1xPBS) (Quality Biological, Gaithersburg, MD; 119-069-131) at room temperature and fixed in 4% paraformaldehyde (PFA) (Thermo Fisher Scientific, Waltham, MA; J19943.K2) in 1xPBS for 1 hour at room temperature, followed by three 15 min washes in 1xPBS with Triton X-100 (Sigma-Aldrich, St. Louis, MO; 9002-93-1) (PBST). .. The specimens were then stained in DAPI (0.5 μg/ml in PBST) (Thermo Fisher Scientific, Waltham, MA; D1306) for 10 min and washed once for 15 min in 1xPBS before mounting with Vectashield antifade mounting media (Vector Laboratories, Newark, CA; H-1000).

    Article Title: Controllable fusion of human brain organoids using acoustofluidics.
    Article Snippet: .. The fixed organoids were then washed twice in 1X phosphate-buffered saline (1XPBS) (Gibco) and transferred to 30% sucrose (w/v) to cryoprotect at 4 °C overnight. ..

    Isolation:

    Article Title: Integrating extracellular vesicle and circulating cell-free DNA analysis on a single plasma aliquot from breast cancer patients improves the detection of HER2 positivity
    Article Snippet: Primary antibodies used were rabbit anti -CD9 (#D801A; Cell signaling Technology; 1/500); rabbit anti – CD 9 (# MA5-33125; Invitrogen; 1/500); mouse anti – Apolipoprotein A1 (#5F4; Cell Signaling Technology; 1/250); rabbit anti– Albumin (#4929; Cell Signaling Technology; 1/1000), mouse anti-Tsg-101 (ab83, Abcam, 1/1000), rabbit anti-Flotillin 1 (#18634, Cell Signaling Technology; 1/1000), rabbit anti-Flotillin 2 (#3436, Cell Signaling Technology; 1/1000), rabbit anti-CD44 (ab157107, Abcam, 1/500), rabbit anti-EpCam (#D1B3, Cell Signaling Technology; 1/500), rabbit anti-HER2/ErbB2 (#29D8, Cell Signaling Technology; 1/1000). .. Isolated EV samples (10 10 -10 11 particles/mL) were resuspended in 1x Phosphate-Buffered Saline (1xPBS) (Gibco) and incubated overnight at 4C with HER2/ErbB2 (29D8) – PE-conjugated antibody (1:50) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (Cell Signaling Technology). .. EVs were then stained with one volume of CellMask plasma membrane stains (C10046, Life Technologies) diluted in 1xPBS (1:5000) for 15 minutes at room temperature, in the dark.

    Incubation:

    Article Title: Integrating extracellular vesicle and circulating cell-free DNA analysis on a single plasma aliquot from breast cancer patients improves the detection of HER2 positivity
    Article Snippet: Primary antibodies used were rabbit anti -CD9 (#D801A; Cell signaling Technology; 1/500); rabbit anti – CD 9 (# MA5-33125; Invitrogen; 1/500); mouse anti – Apolipoprotein A1 (#5F4; Cell Signaling Technology; 1/250); rabbit anti– Albumin (#4929; Cell Signaling Technology; 1/1000), mouse anti-Tsg-101 (ab83, Abcam, 1/1000), rabbit anti-Flotillin 1 (#18634, Cell Signaling Technology; 1/1000), rabbit anti-Flotillin 2 (#3436, Cell Signaling Technology; 1/1000), rabbit anti-CD44 (ab157107, Abcam, 1/500), rabbit anti-EpCam (#D1B3, Cell Signaling Technology; 1/500), rabbit anti-HER2/ErbB2 (#29D8, Cell Signaling Technology; 1/1000). .. Isolated EV samples (10 10 -10 11 particles/mL) were resuspended in 1x Phosphate-Buffered Saline (1xPBS) (Gibco) and incubated overnight at 4C with HER2/ErbB2 (29D8) – PE-conjugated antibody (1:50) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (Cell Signaling Technology). .. EVs were then stained with one volume of CellMask plasma membrane stains (C10046, Life Technologies) diluted in 1xPBS (1:5000) for 15 minutes at room temperature, in the dark.

    Concentration Assay:

    Article Title: Integrating extracellular vesicle and circulating cell-free DNA analysis on a single plasma aliquot from breast cancer patients improves the detection of HER2 positivity
    Article Snippet: Primary antibodies used were rabbit anti -CD9 (#D801A; Cell signaling Technology; 1/500); rabbit anti – CD 9 (# MA5-33125; Invitrogen; 1/500); mouse anti – Apolipoprotein A1 (#5F4; Cell Signaling Technology; 1/250); rabbit anti– Albumin (#4929; Cell Signaling Technology; 1/1000), mouse anti-Tsg-101 (ab83, Abcam, 1/1000), rabbit anti-Flotillin 1 (#18634, Cell Signaling Technology; 1/1000), rabbit anti-Flotillin 2 (#3436, Cell Signaling Technology; 1/1000), rabbit anti-CD44 (ab157107, Abcam, 1/500), rabbit anti-EpCam (#D1B3, Cell Signaling Technology; 1/500), rabbit anti-HER2/ErbB2 (#29D8, Cell Signaling Technology; 1/1000). .. Isolated EV samples (10 10 -10 11 particles/mL) were resuspended in 1x Phosphate-Buffered Saline (1xPBS) (Gibco) and incubated overnight at 4C with HER2/ErbB2 (29D8) – PE-conjugated antibody (1:50) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (Cell Signaling Technology). .. EVs were then stained with one volume of CellMask plasma membrane stains (C10046, Life Technologies) diluted in 1xPBS (1:5000) for 15 minutes at room temperature, in the dark.

    Control:

    Article Title: Integrating extracellular vesicle and circulating cell-free DNA analysis on a single plasma aliquot from breast cancer patients improves the detection of HER2 positivity
    Article Snippet: Primary antibodies used were rabbit anti -CD9 (#D801A; Cell signaling Technology; 1/500); rabbit anti – CD 9 (# MA5-33125; Invitrogen; 1/500); mouse anti – Apolipoprotein A1 (#5F4; Cell Signaling Technology; 1/250); rabbit anti– Albumin (#4929; Cell Signaling Technology; 1/1000), mouse anti-Tsg-101 (ab83, Abcam, 1/1000), rabbit anti-Flotillin 1 (#18634, Cell Signaling Technology; 1/1000), rabbit anti-Flotillin 2 (#3436, Cell Signaling Technology; 1/1000), rabbit anti-CD44 (ab157107, Abcam, 1/500), rabbit anti-EpCam (#D1B3, Cell Signaling Technology; 1/500), rabbit anti-HER2/ErbB2 (#29D8, Cell Signaling Technology; 1/1000). .. Isolated EV samples (10 10 -10 11 particles/mL) were resuspended in 1x Phosphate-Buffered Saline (1xPBS) (Gibco) and incubated overnight at 4C with HER2/ErbB2 (29D8) – PE-conjugated antibody (1:50) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (Cell Signaling Technology). .. EVs were then stained with one volume of CellMask plasma membrane stains (C10046, Life Technologies) diluted in 1xPBS (1:5000) for 15 minutes at room temperature, in the dark.



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